SH2B1 Antibody - BSA Free

Western Blot: SH2B1 Antibody [NBP2-43830] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SH2B1 Antibody [NBP2-43830] - Analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using SH2B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.

Western Blot: SH2B1 Antibody [NBP2-43830] - Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SH2B antibody.

Western Blot: SH2B1 Antibody [NBP2-43830] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SH2B antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SH2B1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SH2B. The exact sequence is proprietary.
• Predicted Species: Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH2B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Application Notes: WB-Ag.
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SH2B1 Antibody - BSA Free

• FLJ30542
• KIAA1299
• Pro-rich, PH and SH2 domain-containing signaling mediator
• PSM
• SH2 domain-containing protein 1B
• SH2 domain-containing putative adapter SH2-B
• SH2B adapter protein 1
• SH2B adaptor protein 1
• SH2-B signaling protein
• SH2B1
• SH2BDKFZp547G1110

Background

The SH2B gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced trans

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SH2B1