SHANK2 Antibody (N23b/6)

Western Blot: SHANK2 Antibody (N23b/6) [NBP2-12914] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6 (NBP2-12914). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12914) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/6) [NBP2-12914] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6 (NBP2-12914). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12914) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/6) [NBP2-12914] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6 (NBP2-12914). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12914) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 Antibody. (D) Composite.

Product Discontinued

SHANK2 Antibody (N23b/6) Summary

• Immunogen: Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (SH3 and multiple ankyrin repeat domains protein 2)
• Localization: Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density , Cell Projection , Growth Cone
• Specificity: Detects approx 160kDa. No cross-reactivity against Shank1 or Shank3. Weak mouse detection.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SHANK2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation
  • Microarray
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of SHANK2 Antibody was sufficient for detection of Shank2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
• Theoretical MW: 159 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for SHANK2 Antibody (N23b/6)

• AUTS17
• cortactin binding protein 1
• cortactin SH3 domain-binding protein
• Cortactin-binding protein 1
• CORTBP1
• CORTBP1CTTNBP1
• CTTNBP1
• GKAP/SAPAP interacting protein
• KIAA1022
• proline-rich synapse associated protein 1
• Proline-rich synapse-associated protein 1
• ProSAP1
• SH3 and multiple ankyrin repeat domains 2
• SH3 and multiple ankyrin repeat domains protein 2
• SHANK
• SHANK2
• SPANK-3

Background

1 ug/mL was sufficient for detection of Shank2 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHANK2 Antibody (NBP2-12914)(1)

Publication using NBP2-12914

• Guerra M, Medici V, Weatheritt R et al. Fetal exposure to valproic acid dysregulates the expression of autism-linked genes in the developing cerebellum Translational psychiatry 2023-04-05 [PMID: 37019889] (WB, Mouse)

Bioinformatics

• Gene Symbol: SHANK2
• Entrez:
  • 171093(Rat)