SHANK3 Antibody - BSA Free

Western Blot: SHANK3 Antibody [NBP3-13167] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK3 antibody (NBP3-13167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13167] - SHANK3 antibody detects SHANK3 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13167) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

• Reactivity: Mu, Rt, Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SHANK3 Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of mouse SHANK3. The exact sequence is proprietary.
• Predicted Species: Human (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHANK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SHANK3 Antibody - BSA Free

• DEL22q13.3
• KIAA1650proline rich synapse associated protein 2
• Proline-rich synapse-associated protein 2
• prosap2
• PSAP2SH3 and multiple ankyrin repeat domains protein 3
• SH3 and multiple ankyrin repeat domains 3
• shank postsynaptic density protein
• Shank3
• SPANK-2

Background

The SHANK3 gene encodes a SH3 and multiple ankyrin repeat domain protein 3 that in isoform 1 is 1,741 amino acids long at 186 kDA and in isoform 2 is 1,732 amino acids long at 185 kDA. This protein functions as an adaptor in postsynaptic density of excitatory synapses. Through complexes that include GKAP/PSD-95 and Homer and the actin-based cytoskeleton, these excitatory synapses are connected with the postsynaptic membrane including NMDA-type and metabotropic glutamate receptor. Additionally, this protein may function in structural and organizational projects of the dendritic spine and the synaptic junction. SHANK3 gene participates in cytoplasmic microtubules, glutamatergic synapses, and signaling events regulated by ret tyrosine kinase. It is known to interact with genes PLCG1, FYN, SRC, ABL1, and PIK3R1. Defects in SHANK3 are associated with autism spectrum disorders and schizophrenia type 15. It is also associated with phelan-mcdermid syndrome, cerebritis, prostatitis, neuronitis, dementia, prostate cancer, hypogonadism, hepatitis a, jacobsen syndrome, and trisomy 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHANK3