SHIP2/INPPL1 Antibody - BSA Free

Western Blot: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of Human and Mouse INPPL2/SHIP2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: SHIP2/INPPL1 Antibody [NB100-1592] - Sample: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 INPPL1/SHIP2 Cat. No. Lot 1 (left) and Lot 2 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of human INPPL2/SHIP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 (lot NB100-1592-2) used for IP at 6 ug per reaction. INPPL2/SHIP2 was also immunoprecipitated by a previous lot of this antibody (NB100-1592-1) and another rabbit anti-INPPL2/SHIP2 antibody. For blotting immunoprecipitated INPPL2/SHIP2, NB100-1592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SHIP2/INPPL1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human inositol polyphosphate phosphatase-like 1 using the numbering given in entry NP_001558.2 (GeneID 3636).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INPPL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Western Blot 1:2000-1:20000
• Application Notes: Epitope retrieval with Citrate buffer pH 6.0 or Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SHIP2/INPPL1 Antibody - BSA Free

• EC 3.1.3
• EC 3.1.3.n1
• inositol polyphosphate phosphatase-like 1
• Inositol polyphosphate phosphatase-like protein 1
• INPPL1
• INPPL-1
• phosphatidylinositol-34,5-trisphosphate 5-phosphatase 2
• Protein 51C
• SH2 domain-containing inositol phosphatase 2
• SH2 domain-containing inositol-5'-phosphatase 2
• SHIP2
• SHIP-2
• SHIP251C protein

Background

INPPL1 (Inositol Polyphosphate Phosphatase-Like Protein 1) is a phosphatidylinositol phosphatase that negatively regulates phosphoinositide 3-kinase (PI3K) pathways related to numerous cell activities. INPPL1 plays a role in PI3K-dependent insulin, macrophage colony-stimulating factor, and EGF signaling. INPPL1 is also involved in actin cytoskeletal remodeling, actin dynamics, and neuritogenesis. INPPL1 interacts with SHC1 (src-homology domain) to mediate EGFR signaling. Alternate names for INPPL1 include SH2 domain-containing inositol-5'phosphatase 2, SHIP-2, INPPL-1, and protein 51C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHIP2/INPPL1 Antibody (NB100-1592)(1)

Publication using NB100-1592

• Yoshinaga S, Ohkubo T, Sasaki S et al. A phosphatidylinositol lipids system, lamellipodin, and Ena/VASP regulate dynamic morphology of multipolar migrating cells in the developing cerebral cortex. J Neurosci 2012-08-01 [PMID: 22915108]

Bioinformatics

• Gene Symbol: INPPL1
• Entrez:
  • 3636(Human)
• Uniprot:
  • NP_001558.2(Human)