SHP-2/PTPN11 Antibody - BSA Free

Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP-2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NBP1-33064] - Paraffin-embedded BT483 xenograft.SHP2 antibody dilution: 1:500.

Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE, antibody diluted at 1:500.

Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - 50 ug rat brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.

Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NBP1-33064] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM, Xp
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SHP-2/PTPN11 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human SHP-2/PTPN11. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Bovine (98%), Rhesus Macaque (100%), Chicken (98%), Xenopus (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PTPN11
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SHP-2/PTPN11 Antibody - BSA Free

• BPTP3
• EC 3.1.3.48
• MGC14433
• protein tyrosine phosphatase, non-receptor type 11
• protein tyrosine phosphatase-2
• Protein-tyrosine phosphatase 1D
• Protein-tyrosine phosphatase 2C
• PTP1D
• PTP-1D
• PTP2C
• PTP-2C
• PTP2CNS1
• PTPN11
• SHP2
• SHP-2
• SHP2CFC
• SHPTP2
• SH-PTP2
• SH-PTP2Noonan syndrome 1
• SH-PTP3
• tyrosine-protein phosphatase non-receptor type 11

Background

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PTPN11
• Entrez:
  • 5781(Human)