![Western Blot: TAP Tag Antibody [NBP2-31052] - Analysis of 10 uL of yeast extract and 7ul of Molecular Weight Protein Ladder per well](http://images.novusbio.com/fullsize/TAP-Tag-Antibody-Western-Blot-NBP2-31052-img0003.jpg "Western Blot: TAP Tag Antibody [NBP2-31052] - Analysis of 10 uL of yeast extract and 7ul of Molecular Weight Protein Ladder per well")
| Reactivity | Hu, All-NA, YeSpecies Glossary |
| Applications | WB, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 0.5 mg/ml |
| Immunogen | KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C in the dark. |
| Buffer | BBS (pH 8.4) |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: TAP Tag Antibody [NBP2-31052] - High-expressing (clone 1) and low expressing (clone 2) TAP fusion proteins probed with 0.5ug/ml of anti-TAP antibody for 2 hours at RT under standard western blotting conditions. The size of the band varies depending on the size of the fusion protein TAP adds around 20kDa to the size of the protein.](http://images.novusbio.com/fullsize/TAP-Tag-Antibody-Western-Blot-NBP2-31052-img0001.jpg "Western Blot: TAP Tag Antibody [NBP2-31052] - High-expressing (clone 1) and low expressing (clone 2) TAP fusion proteins probed with 0.5ug/ml of anti-TAP antibody for 2 hours at RT under standard western blotting conditions. The size of the band varies depending on the size of the fusion protein TAP adds around 20kDa to the size of the protein.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")