TIM-1/KIM-1/HAVCR Antibody - BSA Free

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - In vitro nephrotoxicity assessment of kidney spheroids. Western blotting analysis of kidney injury markers in kidney spheroids after treatment with 20 uM cisplatin (CPT) or 50 uM cisplatin cyclosporin A (CSA). All data are shown as the means +/- s.e.m. *p < 0.05 compared with control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Analysis of human uterus tissue using anti-TIM-1 antibody (3809) at 10 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP)at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), overnightincubation at 4C (3809) or 1h incubation at RT in 5% NFDM/TBST. Secondary:Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. Antibodies: 3809 (8 ug/mL), 1 hincubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 u of lysates per lane.Antibodies: TIM-1 (8 u/mL), overnight incubation at 4; C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody - BSA Free [NBP1-76701] - Validation of TIM-1/KIM-1/HAVCR in Human Uterus Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1/KIM-1/HAVCR with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TIM-1/KIM-1/HAVCR Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1. Amino Acid Squence: TNGTHVTYRKDTRYKL
• Specificity: Human TIM-1/KIM-1/HAVCR has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD). This antibody detects human isoform b precursor. Mouse TIM-1/KIM-1/HAVCR has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1/KIM-1/HAVCR has only one isoform identified so far (307aa, 34kD).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAVCR1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Knockdown Validated
  • Simple Western 40 ug/mL
  • Western Blot 1-8 ug/ml
• Application Notes: Use in IF reported in scientific literature (PMID:33644078). ICC/IF reactivity reported in (PMID: 27214555).
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TIM-1/KIM-1/HAVCR Peptide (NBP1-76701PEP)

Reactivity Notes

Mouse reactivity reported in (PMID: 29369823). Rat reactivity reported in (PMID: 30066041).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TIM-1/KIM-1/HAVCR Antibody - BSA Free

• CD365
• HAVCR1
• HAVCR-1
• HAVCRT cell immunoglobin domain and mucin domain protein 1
• hepatitis A virus cellular receptor 1
• Kidney injury molecule 1
• KIM1
• KIM-1
• T-cell immunoglobulin and mucin domain-containing protein 1
• TIM1
• TIM-1
• TIM-1TIM
• TIM1TIMD-1
• TIMD1T-cell membrane protein 1

Background

The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-1/KIM-1/HAVCR Antibody (NBP1-76701)(37)

Publications using NBP1-76701

• Bae, CR;Kim, Y;Kwon, YG; CU06-1004 alleviates oxidative stress and inflammation on folic acid-induced acute kidney injury in mice Journal of pharmacological sciences 2024-02-01 [PMID: 38246731]
• Çomakl? S, Özdemir S, Gülo?lu M Chrysin attenuates paclitaxel-induced hepatorenal toxicity in rats by suppressing oxidative damage, inflammation, and apoptosis Life sciences 2023-09-15 [PMID: 37716503] (Rat)
• An S, Yao Y, Wu J et al. Gut-derived 4-hydroxyphenylacetic acid attenuates sepsis-induced acute kidney injury by upregulating ARC to inhibit necroptosis Biochimica et biophysica acta. Molecular basis of disease 2023-09-13 [PMID: 37714058]
• Yang CC, Chen YL, Sung PH et al. Repeated administration of adipose-derived mesenchymal stem cells added on beneficial effects of empagliflozin on protecting renal function in diabetic kidney disease rat Biomedical journal 2023-06-22 [PMID: 37355087] (ICC/IF, IHC, Rat)
• Ban K, Nam G, Kim D et al. Prevention of LPS-Induced Acute Kidney Injury in Mice by Bavachin and Its Potential Mechanisms Antioxidants 2022-10-24 [PMID: 36358467] (IHC-P, Mouse)
• Eliasson Angelstig S Mechanistic studies on the repression of cellular reaction to hypoxia as cause for chronic complications in diabetes Thesis 2021-01-01 (WB, Human)
• Micha?ek K, Oberska P, Murawski M et al. Kidney morphology and renal expression of aquaporins 2, 3 and 4 during cerulein - Induced chronic pancreatitis in pigs Advances in medical sciences 2023-09-12 [PMID: 37708639] (WB, IHC-Fr, Porcine)
• Wu T, Zhu W, Duan R et al. Magnetic vagus nerve stimulation ameliorates contrast-induced acute kidney injury by circulating plasma exosomal miR-365-3p Journal of Nanobiotechnology 2024-10-28 [PMID: 39468562]
• Dong Y, Jia Z, Kang B et Al. Annexin-A5 monomer as a membrane repair agent for the treatment of renal ischemia-reperfusion injury Mol Biol Rep 2024-05-25 [PMID: 38796668]
• Zhong, D;Chen, J;Qiao, R;Song, C;Hao, C;Zou, Y;Bai, M;Su, W;Yang, B;Sun, D;Jia, Z;Sun, Y; Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erb?/FABP5 signaling Cell reports 2024-04-06 [PMID: 38583151]
• Li H, Duan J, Zhang T et Al. miR-16-5p aggravates sepsis-associated acute kidney injury by inducing apoptosis Ren Fail 2024-03-06 [PMID: 38445373]
• Huang L, Zhang L, Zhang Z et Al. Loss of nephric augmenter of liver regeneration facilitates acute kidney injury via ACSL4-mediated ferroptosis J Cell Mol Med 2023-12-13 [PMID: 38088220]
• Kansakar U, Gambardella J, Varzideh F et al. miR-142 Targets TIM-1 in Human Endothelial Cells: Potential Implications for Stroke, COVID-19, Zika, Ebola, Dengue, and Other Viral Infections International Journal of Molecular Sciences 2022-09-06 [PMID: 36142146] (Immunohistochemistry, Western Blot, Mouse)
• Freitas F, Attwell D Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemia eLife 2022-03-14 [PMID: 35285797] (IF/IHC, Rat)
• Zheng X, Narayanan S, Xu C et al. Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes eLife 2022-02-15 [PMID: 35164902] (IHC-P, Mouse)
• Liu HH, Li XQ, Liu JF et al. miR-6869-5p Transported by Plasma Extracellular Vesicles Mediates Renal Tubule Injury and Renin-Angiotensin System Activation in Obesity Frontiers in Medicine 2021-09-08 [PMID: 34568382] (Immunohistochemistry, Western Blot, Mouse)
• Sun M, Li J, Mao L et al. p53 Deacetylation Alleviates Sepsis-Induced Acute Kidney Injury by Promoting Autophagy Frontiers in Immunology 2021-07-14 [PMID: 34335587] (IHC-P)
• Yang C, Zhang Y, Zeng X et al. Kidney injury molecule-1 is a potential receptor for SARS-CoV-2 Journal of Molecular Cell Biology 2021-01-25 [PMID: 33493263] (Immunohistochemistry, Western Blot, Mouse)
• Samodelov SL, Visentin M, Gai Z et al. Renal glycosuria as a novel early sign of colistin-induced kidney damage in mice Antimicrob. Agents Chemother. 2019-10-07 [PMID: 31591120] (IF/IHC, Mouse)
• Qing X, Chinenov Y, Redecha P et al. iRhom2 promotes lupus nephritis through TNF-a and EGFR signaling J. Clin. Invest. 2018-01-25 [PMID: 29369823] (Mouse)
• Trudu M, Schaeffer C, Riba M et al. Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations Sci Rep 2017-08-07 [PMID: 28785050] (WB, Mouse)
• Tunbenjasiri K, Pongking T, Sitthirach C et al. Alterations of metagenomics and metaproteomics associate kidney disease in a combination of opisthorchiasis and nonalcoholic fatty liver disease bioRxiv 2023-09-23 (IHC, Mouse)
• Han S, Lin F, Ruan Y Et Al. miR-132-3p promotes the cisplatin-induced apoptosis and inflammatory response of renal tubular epithelial cells by targeting SIRT1 via the NF-kappaB pathway International immunopharmacology 2021-07-30 [PMID: 34339961] (IHC-P)
• Zhang Q, Wang L, Wu M et al. Humanized anti TLR4 monoclonal antibody ameliorates lipopolysaccharide related acute kidney injury by inhibiting TLR4/NF kappa B signaling Molecular medicine reports 2021-08-01 [PMID: 34184086] (Mouse)
• Zheng H, Zhang Y, Li L et al. Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury Frontiers in Cell and Developmental Biology 2021-02-12 [PMID: 33644078] (IF, IF/IHC, Mouse)
• Kang HM, Lim JH, Noh KH et al. Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies Sci Rep 2019-11-26 [PMID: 31772214] (ICC/IF, Mouse)
• Yao Z, Qiao Y et al. Exosomes Exploit the Virus Entry Machinery and Pathway To Transmit Alpha Interferon-Induced Antiviral Activity. J Virol 2018-12-15 [PMID: 30282711] (IF/IHC, Human)
• Song MF, Yang Y, Yi ZW et al. Sema 3A as a biomarker of the activated mTOR pathway during hexavalent chromium-induced acute kidney injury. Toxicol. Lett. 2018-09-17 [PMID: 30236763] (WB, Human)
• Sharma M, Naura AS, Singla SK. Modulatory effect of 4-phenyl butyric acid on hyperoxaluria-induced renal injury and inflammation Mol. Cell. Biochem. 2018-07-31 [PMID: 30066041] (IHC-P, Rat)
• Morrison MC, Yakala GK, Liang W et al. Protective effect of rosiglitazone on kidney function in high-fat challenged human-CRP transgenic mice: a possible role for adiponectin and miR-21. Sci Rep. 2017-06-06 [PMID: 28588299] (IF/IHC, Mouse)
• Weber HM, Tsurkan MV, Magno V et al. Heparin-based hydrogels induce human renal tubulogenesis in vitro. Acta Biomater 2017-05-17 [PMID: 28526628]
• Pei L, Solis G, Nguyen MT et al. Paracellular epithelial sodium transport maximizes energy efficiency in the kidney J. Clin. Invest 2016-07-01 [PMID: 27214555] (ICC/IF, Mouse)
• Tang W H Wilson, Wang Zeneng, Kennedy David J et al. Gut microbiota-dependent trimethylamine N-oxide (TMAO) pathway contributes to both development of renal insufficiency and mortality risk in chronic kidney disease. Circulation Research 2015-01-01 [PMID: 25599331] (WB, Mouse)
• Trudu M, Janas S, Lanzani C et al. Common noncoding UMOD gene variants induce salt-sensitive hypertension and kidney damage by increasing uromodulin expression. Nat Med. 2013-12-01 [PMID: 24185693] (WB, Mouse)
• Lim A,Chan L, Lai KN et al. Distinct role of matrix metalloproteinase-3 in kidney injury molecule-1 shedding by kidney proximal tubular epithelial cells The International Journal of Biochemistry & Cell Biology 2012-03-27 [PMID: 22484054] (WB, IF/IHC, ICC/IF, Mouse, Human)
• Sorensen I, Rong S, Susnik N et al. Bbeta(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation J Am Soc Nephrol 2011-10-01 [PMID: 21841063] (IF/IHC, WB, Mouse)

FAQs for TIM-1/KIM-1/HAVCR Antibody (NBP1-76701). (Showing 1 - 1 of 1 FAQs).

1. I am using  KIM-1 antibody NBP1-76701 on NIH-3T3 cell lysate. What is the molecular weight of KIM1? Also, do you suggest any positive control cell line or tissue for WB testing of KIM1?
   • The predicted MWt of KIM-1 is ~38.7 kDa and in our Western Blot validation of TIM-1/KIM-1/HAVCR antibody [NBP1-76701] at 1 ug/mL dilution, we observed a specific band around this position in 3T3 cell lysate. The other cell type which is known to express KIM1 is stimulated T-cells wherein it is expressed during primary antigen stimulation (PMID 11725301). According to protein atlas database (ENSG00000113249), this protein is expressed in Kidney & gastrointestinal tissues also. 

Bioinformatics

• Gene Symbol: HAVCR1
• Entrez:
  • 26762(Human)
• Uniprot:
  • Q96D42()