TMEM97 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TMEM97 Antibody [NBP1-30436] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TMEM97 Antibody NBP1-30436 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: TMEM97 Antibody [NBP1-30436] - Expression of proteins involved in extracellular lipid uptake and intracellular cholesterol trafficking following JQ1 (0.4 uM) administration to HepG2 cells for 48 hours. Representative Western blots and densitometric analysis of TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means +/- SD. Statistical analysis was performed by using unpaired Student's t test. ** p < 0.01; *** p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/21/4/1297), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP1-30436] - Analysis of a FFPE tissue section of human pancreas using 1:200 dilution of TMEM97 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Western Blot: TMEM97 Antibody [NBP1-30436] - Analysis of TMEM97 in HeLa whole cell extracts.

• Reactivity: Hu, Mu, Pm
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TMEM97 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of the human TMEM97 protein (within residues 105-155. [Swiss-Prot: Q5BJF2]
• Localization: Membrane; Multi-pass membrane protein
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM97
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:50
  • Western Blot 2 ug/ml
• Application Notes: In Western blot, a band is seen at ~24 kDa.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

• Control Peptide:

  TMEM97 Peptide (NBP1-30436PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: crab-eating macaque (98%). Mouse reactivity reported in scientific literature (PMID: 30594810).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMEM97 Antibody - BSA Free

• anti
• MAC30
• MAC30Protein MAC30
• S2R
• Sigma2 receptor
• Sigma-2 receptor
• TMEM97
• transmembrane protein 97

Background

TMEM97 (transmembrane protein 97 or protein MAC30) is a member of IGFBP/insulin-like growth factor-binding protein family and is implicated in several biological processes wherein it acts through its regulatory effects on IGF activity. It is a multi-pass membrane protein which is localized in nuclear and cell membranes, RER and lysosomes, and is expressed in several normal tissues including brain, lung, heart, skeletal muscles, testis, ovary, and pregnant uterus. TMEM97 was originally identified as overexpressed in meningiomas, however, later it was found to exhibit altered expression in different types of cancers. The absence or downregulation of TMEM97 expression in pancreatic cancer suggests that it may acts as a tumor suppressor in pancreatic cancer. Contrastingly, its expression is stronger in breast, esophageal, stomach, colon, and oral squamous cell cancers than the corresponding normal tissues. Its physiological function is largely unknown, however, it has been suggested to implicate in cellular proliferation/differentiation and regulation of cholesterol homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMEM97 Antibody (NBP1-30436)(12)

Publications using NBP1-30436

• Sorbi C, Belluti S, Atene CG et al. BS148 Reduces the Aggressiveness of Metastatic Melanoma via Sigma-2 Receptor Targeting International journal of molecular sciences 2023-06-02 [PMID: 37298633] (WB, Human)
• Thejer BM, Infantino V, Santarsiero A et al. Sigma-2 Receptor Ligand Binding Modulates Association between TSPO and TMEM97 International journal of molecular sciences 2023-03-28 [PMID: 37047353] (WB)
• Mao D, Zhang X, Wang Z et al. TMEM97 is transcriptionally activated by YY1 and promotes colorectal cancer progression via the GSK-3 beta/beta-catenin signaling pathway Human cell 2022-07-30 [PMID: 35907137]
• Liu CZ, Mottinelli M, Nicholson HE et al. Identification and characterization of MAM03055A: a novel bivalent sigma-2 receptor/TMEM97 ligand with cytotoxic activity European journal of pharmacology 2021-06-15 [PMID: 34144027]
• Sereti E, Tsimplouli C, Kalaitsidou E, et al. Study of the Relationship between Sigma Receptor Expression Levels and Some Common Sigma Ligand Activity in Cancer Using Human Cancer Cell Lines of the NCI-60 Cell Line Panel Biomedicines 2021-01-05 [PMID: 33466391] (WB, Human)
• Riad A, Lengyel-Zhand Z, Zeng C et Al. The Sigma-2 Receptor/TMEM97, PGRMC1, and LDL Receptor complex are responsible for the cellular uptake of A beta 42 and its protein aggregates Mol Neurobiol 2020-06-24 [PMID: 32572762]
• Ryskamp D, Wu L, Wu J et al. Pridopidine stabilizes mushroom spines in mouse models of Alzheimer's disease by acting on the sigma-1 receptor Neurobiol. Dis. 2018-12-27 [PMID: 30594810] (WB, Mouse)
• Riad A, Zeng C, Weng CC et al. Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex. Sci Rep. 2018-11-15 [PMID: 30443021] (ICC/IF, Human)
• Ebrahimi-Fakhari D, Wahlster L, Bartz F et al. Reduction of TMEM97 increases NPC1 protein levels and restores cholesterol trafficking in Niemann-pick type C1 disease cells Hum. Mol. Genet. 2016-07-04 [PMID: 27378690] (ICC/IF)
• Xu XY, Zhang LJ, Yu YQ et al. Down-Regulated MAC30 Expression Inhibits Proliferation and Mobility of Human Gastric Cancer Cells. Cell. Physiol. Biochem. 2014-05-05 [PMID: 24853233] (ICC/IF, WB, Human)
• Shen H, Li J, Xie X et al. BRD2 regulation of sigma-2 receptor expression upon cytosolic cholesterol deprivation bioRxiv (WB)
  
  Details:
  WB concentration 1:200
• Tonini C, Colardo M, et al. Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis. Int J Mol Sci 2020-02-14 [PMID: 32075110] (WB, Human)

Bioinformatics

• Gene Symbol: TMEM97
• Entrez:
  • 27346(Human)
  • 69071(Mouse)
• Uniprot:
  • Q5BJF2(Human)
  • Q8VD00(Mouse)