TROP-2 Antibody - BSA Free

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP2-49166] - Analysis in human skin and liver tissues. Corresponding TROP-2 RNA-seq data are presented for the same tissues.
Orthogonal Strategies: Western Blot: TROP-2 Antibody [NBP2-49166] - Analysis in human cell lines A-431 and HEK293 using Anti-TACSTD2 antibody. Corresponding TACSTD2 RNA-seq data are presented for the same cell ...read more
Immunocytochemistry/ Immunofluorescence: TROP-2 Antibody [NBP2-49166] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli & plasma membrane.
Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP2-49166] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP2-49166] - Staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP2-49166] - Staining of human liver shows no positivity in hepatocytes, as expected.
Immunohistochemistry-Paraffin: TROP-2 Antibody [NBP2-49166] - Staining of human prostate shows strong membranous positivity in glandular cells.
Flow Cytometry: Rabbit Polyclonal TROP-2 Antibody [NBP2-49166] - Detection of TROP-2 on CHOK1 cells expressing human TROP-2. Cells received 5 uL of the human TROP-2 antibody (BLUE) or isotype control (RED) for 30 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Validated by:
       

Orthogonal Strategies

 

Order Details

TROP-2 Antibody - BSA Free Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: AFNHSDLDAELRRLFRERYRLHPKFVAAVHYEQPTIQIELRQNTSQKAAGEVDIGDAAYYFERDIKGESL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TACSTD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry Validated for Flow Cytometry from a verified customer review.
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TROP-2 Recombinant Protein Antigen (NBP2-49166PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-49166 in the following applications:

Reactivity Notes

Mouse (81%), Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TROP-2 Antibody - BSA Free

  • Cell surface glycoprotein Trop-2
  • EGP1
  • EGP-1
  • epithelial glycoprotein-1
  • GA733-1
  • GA733-1GP50
  • gastrointestinal tumor-associated antigen GA7331
  • gp50
  • M1S1cell surface glycoprotein TROP2
  • Membrane component chromosome 1 surface marker 1
  • membrane component, chromosome 1, surface marker 1
  • Pancreatic carcinoma marker protein GA733-1
  • pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733
  • T16
  • TACSTD2
  • TROP2
  • TROP-2
  • TROP2GA7331
  • tumor-associated calcium signal transducer 2

Background

TROP2 encodes a carcinoma-associated antigen defined by the monoclonal antibody GA733. This antigen is a member of a family including at least two type I membrane proteins. It transduces an intracellular calcium signal and acts as a cell surface receptor. Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TROP-2 Antibody (NBP2-49166) (0)

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Review for TROP-2 Antibody (NBP2-49166) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-49166:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry TROP-2 NBP2-49166
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5
reviewed by:
Panagiotis Fotakis
Flow Human 02/15/2024
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Summary

ApplicationFlow Cytometry
Sample TestedCHO cells expressing human Trop2,CHOK1 expressing hTrop2,CHOK1 expressing human TROP2
SpeciesHuman
Lot000004774

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TROP-2 Antibody (NBP2-49166) (0)

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Recent Reviews

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1
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Panagiotis Fotakis
02/15/2024
Application: Flow
Species: Human

Bioinformatics

Gene Symbol TACSTD2