VDAC2 Antibody - BSA Free

Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP1-89477] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

VDAC2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MATHGQTCARPMCIPPSYADLGKAAR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VDAC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  VDAC2 Protein (NBP1-89477PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for VDAC2 Antibody - BSA Free

• FLJ23841
• hVDAC2
• Outer mitochondrial membrane protein porin 2
• POR
• VDAC-2
• voltage-dependent anion channel 2
• voltage-dependent anion-selective channel protein 2

Background

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Interacts with hexokinases. It is present in the outer mitochondrial membrane. There are 4 isoforms as result of alternative splicing. Expressed in all tissues examined. Consists mainly of membrane spanning sided beta sheets. Belongs to the eukaryotic mitochondrial porin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VDAC2 Antibody (NBP1-89477)(2)

Publications using NBP1-89477

• Belcher BP, Machicao PA, Tong B et al. Chemoproteomic Profiling Reveals that Anti-Cancer Natural Product Dankastatin B Covalently Targets Mitochondrial VDAC3 bioRxiv : the preprint server for biology 2023-02-11 [PMID: 36798342] (Human)
• Belcher BP Chemoproteomic Strategies for Drug Discovery and Natural Product Target Identification Thesis 2023-01-01
  
  Details:
  Gel-Based ABPP

Bioinformatics

• Gene Symbol: VDAC2
• Entrez:
  • 7417(Human)
• Uniprot:
  • P45880(Human)