AFAP Antibody (OTI2G9) [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: OTI2G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

AFAP Antibody (OTI2G9) [Alexa Fluor® 532] Summary

• Immunogen: Recombinant protein fragment corresponding to amino acids 100-386 of human AFAP (NP_940997) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AFAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for AFAP Antibody (OTI2G9) [Alexa Fluor® 532]

• actin filament associated protein 1
• AFAP
• AFAP1
• AFAP-110
• FLJ56849

Background

AFAP, or Actin filament-associated protein 1, contains a 81 kDa and a 90 kDa isoform, and is involved in the manipulation of actin filaments to form network or bundle structures and the regulation of cell-matrix linkage. Disease research is currently being conducted with AFAP and its relation to familial adenomatous polyposis, adenocarcinoma, breast cancer, prostate adenocarcinoma, prostatitis, polyposis, prostate cancer, and carcinoma. This protein interacts with PRKCB, PRKCD, PRKCG, ACTC1, and PRKCZ during the processes of signal transduction regulation and mechanical stimulus response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFAP1