AFAP1L2 Antibody - BSA Free

Western Blot: AFAP1L2 Antibody [NBP1-77358] - Mouse liver tissue lysate with AFAP1L2 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: AFAP1L2 Antibody - BSA Free [NBP1-77358] - Immunohistochemistry of AFAP1L2 in human liver tissue with AFAP1L2 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: AFAP1L2 Antibody - BSA Free [NBP1-77358] - Immunofluorescence of AFAP1L2 in human liver tissue with AFAP1L antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AFAP1L2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AFAP1L2. The immunogen is located within the last 50 amino acids of AFAP1L2. Amino Acid Squence: TVLQKAKEWEKKGAS
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AFAP1L2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  AFAP1L2 Peptide (NBP1-77358PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AFAP1L2 Antibody - BSA Free

• actin filament associated protein 1-like 2
• actin filament-associated protein 1-like 2
• AFAP1-like protein 2
• FLJ14564
• KIAA1914Em:AC005383.4
• XB130CTB-1144G6.4

Background

AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFAP1L2
• Uniprot:
  • NP_001001936(Human)