c-Fos Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 4 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for c-Fos at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

c-Fos was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human c-Fos Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7254) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

c-Fos Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human c-Fos
  Met1-Leu132
  Accession # P01100
• Specificity: Detects human c-Fos in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Fos-B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FOS
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for c-Fos Antibody [Unconjugated]

• activator protein 1
• AP-1
• cellular oncogene c-fos
• Cellular oncogene fos
• cFos
• c-Fos
• FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
• FBJ murine osteosarcoma viral oncogene homolog
• Fos proto-oncogene, AP-1 trancription factor subunit
• FOS
• G0/G1 switch regulatory protein 7
• G0S7
• p55
• proto-oncogene c-Fos

Background

Cellular oncogene fos (c-Fos) is one of four Fos family proteins that associate with Jun family proteins to form the AP-1 transcription factor complex. It is thought to have an important role in signal transduction, cell proliferation and differentiation. Cellular oncogene fos (c-Fos) is constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Within aa 1-132, human c-Fos shares 92% aa sequence identity with mouse and rat c-Fos.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for c-Fos Antibody (AF7254)(3)

Publications using AF7254

• Hongshu Chen, Lijuan Ji, Xiuzhen Liu, Jihong Zhong Correlation between the rs7101 and rs1063169 polymorphisms in the FOS noncoding region and susceptibility to and prognosis of colorectal cancer Medicine (Baltimore) 2019-07-03 [PMID: 31261535] (Western Blot, Human)
• Hongshu Chen, Lijuan Ji, Xiuzhen Liu, Jihong Zhong Correlation between the rs7101 and rs1063169 polymorphisms in the FOS noncoding region and susceptibility to and prognosis of colorectal cancer Medicine (Baltimore) 7/3/2019 [PMID: 31261535]
• Hayashida C, Ito J, Nakayachi M, Okayasu M, Ohyama Y, Hakeda Y, Sato T Osteocytes produce interferon-beta as a negative regulator of osteoclastogenesis. J Biol Chem, 2014-03-07;289(16):11545-55. 2014-03-07 [PMID: 24610813] (Western Blot, Mouse)

FAQs for c-Fos Antibody (AF7254). (Showing 1 - 1 of 1 FAQs).

1. I am looking for some c-fos antibodies that could work in IHC.
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Bioinformatics

• Gene Symbol: FOS
• Uniprot:
  • P01100(Human)