CDCA3 Antibody (SR1419) - BSA Free

Western Blot: CDCA3 Antibody (SR1419) [NBP3-21698] - Western blot analysis using NBP3-21698 in MCF7 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: SR1419
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDCA3 Antibody (SR1419) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human CDCA3 (Uniprot #: Q99618)
• Specificity: Detects endogenous levels of total CDCA3
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDCA3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDCA3 Antibody (SR1419) - BSA Free

• C8
• cell division cycle associated 3
• FBXO52
• Gene-rich cluster protein C8
• GRCC8cell division cycle-associated protein 3
• MGC2577
• TOME1
• TOME-1gene rich cluster, C8
• trigger of mitotic entry 1
• Trigger of mitotic entry protein 1

Background

CDCA3/TOME1 is an F-box protein and is part of the SCF complex, an ubiquitin ligase complex that promotes degradation of the mitosis-inhibitory kinase, wee1. CDCA3/TOME1 expression is cell cycle regulated by cis-promoter elements and activated at the G2/M phase. CDCA3/TOME1 levels are also regulated at the protein level via degradation by the APC (anaphase-promoting complex) during G1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDCA3