GPER/GPR30 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human breast carcinoma.Tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with breast carcinoma.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human, pancreas, islet.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Analysis of anti-GPER1 / GPR30 antibody with human adrenal.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human colon, carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Anti-GPER1 / GPR30 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, Parkinson's Lewy Body

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS1183] - Brain, cerebellum, Purkinje neuron

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPER/GPR30 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPER/GPR30.
• Epitope: 1st cytoplasmic domain
• Localization: Endoplasmic reticulum - multi-pass membrane protein.
• Specificity: Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 15 - 20 ug/ml
  • Western Blot 1:1000 - 1:10000
• Application Notes: ICC/IF usage is reported in scientific literature (PMID: 24011568). Use in IHC reported in scientific literature (PMID: 24011568)..
• Control:

  MCF-7 Whole Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (Natale C et al). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (94%), Monkey (88%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPER/GPR30 Antibody - BSA Free

• CEPR
• CEPRG-protein coupled receptor 30
• Chemoattractant receptor-like 2
• chemokine receptor-like 2
• CMKRL2
• CMKRL2MGC99678
• constitutively expressed peptide-like receptor
• DRY12
• DRY12IL8-related receptor DRY12
• FEG-1
• FEG-1mER
• Flow-induced endothelial G-protein coupled receptor 1
• G protein-coupled estrogen receptor 1
• G protein-coupled receptor 30
• GPCR-BR
• GPER
• GPR30
• GPR30GPCR-Br
• G-protein coupled estrogen receptor 1
• heptahelix receptor
• LERGU
• LERGU2
• leucine rich protein in GPR30 3'UTR
• LyGPR
• Lymphocyte-derived G-protein coupled receptor
• Membrane estrogen receptor

Background

GPR30 a former orphan receptor has been linked to estrogen-mediated activation of adenylyl cyclase, release of epidermal growth factor (EGF)-related ligands, and specific estrogen binding.1 GPR30 acts independently of estrogen receptors, ERalpha and ERbeta, to release of heparin-binding EGF and may play a role in breast cancer tumorogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPER/GPR30 Antibody (NLS1183)(6)

Publications using NLS1183

• Spatkowska M, Dyduch G, Broniatowska E Et al. Molecular Proof of a Clinical Concept: Expression of Estrogen Alpha-, Beta-Receptors and G Protein-Coupled Estrogen Receptor 1 (GPER) in Histologically Assessed Common Nevi, Dysplastic Nevi and Melanomas Medicina (Kaunas, Lithuania) 2021-11-11 [PMID: 34833446] (IHC-P, Human)
• Natale CA, Li J, Pitarresi JR et al. Pharmacologic Activation of the G Protein-Coupled Estrogen Receptor Inhibits Pancreatic Ductal Adenocarcinoma Cell Mol Gastroenterol Hepatol 2020-05-04 [PMID: 32376419] (IHC-P)
• Natale CA, Li J, Zhang J et al. Activation of G protein-coupled estrogen receptor signaling inhibits melanoma and improves response to immune checkpoint blockade Elife 2018-01-16 [PMID: 29336307] (IHC-P, Human)
• Steiman J, Peralta EA, Louis S, Kamel O. Biology of the estrogen receptor, GPR30, in triple negative breast cancer. Am J Surg. 2013-09-04 [PMID: 24011568] (IF/IHC, ICC/IF, Human)
• Hoffmeister MV, Damkier HH, Christensen BM et al. 17{beta}-estradiol induces non-genomic effects in renal intercalated cells through the G-protein coupled estrogen receptor 1. Am J Physiol Renal Physiol. 2011-10-12 [PMID: 21993891]
• Park II, Zhang Q, Liu V et al. 17{beta}-estradiol at low concentrations acts through distinct pathways in normal versus BPH-derived prostate stromal cells. Endocrinology :en.2008-1591. 2009-01-01 [PMID: 19608654]

Reviews for GPER/GPR30 Antibody (NLS1183) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 12/29/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 24011568
• Species: Human

reviewed by: Verified Customer IHC Human 09/14/2010

Summary

• Application: Immunohistochemistry
• Sample Tested: Human breast tissue
• Species: Human

Bioinformatics

• Gene Symbol: GPER1
• Entrez:
  • 2852(Human)
• Uniprot:
  • Q99527()