GPER/GPR30 Antibody - BSA Free

Western Blot: GPER/GPR30 Antibody [NLS4271] - Direct association of dopamine transporter and estrogen receptors on the plasma membrane vs. in total protein. Co-immunoprecipitation of dopamine transporter after 9 min 10-9 M E2 treatment and subsequent probing with antibodies for DAT, ERalpha, ERbeta, and GPR30. This is a representative immunoblot of 3 experiments Image collected and cropped by CiteAb from the following publication (//www.biomedcentral.com/1471-2202/10/59), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with human brain, hippocampus.

Western Blot: GPER/GPR30 Antibody [NLS4271] - Analysis of anti-GPR30 antibody with rat hypothalamus 5-10ug protein loaded per lane. Image courtesy provided by Carrie McAllister.

Direct association of dopamine transporter and estrogen receptors on the plasma membrane vs. in total protein. Co-immunoprecipitation of dopamine transporter after 9 min 10-9 M E2 treatment and subsequent probing with antibodies for DAT, ER alpha , ER beta , and GPR30. This is a representative immunoblot of 3 experiments

Western Blot: GPER/GPR30 Antibody - BSA Free [NLS4271] - GPER/GPR30 expression in normal lung cell lines & lung adenocarcinoma cell lines. A) The expression of GPER was determined by realtime quantitative PCR in each of the normal lung cell lines (open bars), lung adenocarcinoma cell lines (grey bars), & MCF-7 human breast cancer cells (black bar). Values are the average of 4–6 biological replicates ± SEM. B-D) Representative western blot analysis of GPER/GPR30 expression in the indicted cell lines. 30 μg of whole cell extract (WCE) were separated on 10% SDS PAGE gels with MW marker migration indicated at the left of each blot. The diagrams at the top of B-C, & D indicate the region of GPER recognized by the 3 different polyclonal antibodies used: B) Novus NBP-1-31239, C) Novus NLS4271; D) Santa Cruz sc-4854-R. The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation & interaction with other proteins. Bands are identified as glycosylated & nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped & reprobed for beta -actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands & dividing by beta -actin, then normalizing to HBEC2-KT in each blot. Panel E is a summary of all westerns (not all westerns are shown) with each antibody. For Westerns with NBP1-31239 & sc-48524, values are the mean ± SEM from 3 separate blots using different WCE. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23273253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm, Ca, Ha, Pm
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GPER/GPR30 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPER/GPR30.
• Epitope: 3rd extracellular domain
• Localization: Endoplasmic reticulum - multi-pass membrane protein.
• Specificity: Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Canine (94%), Hamster (94%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GPER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 6.4 ug/ml
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: Antibody does not work in Western Blot for mouse samples. IP usage is reported in scientific literature (PMID: 19531209). ELISA usage is reported in scientific literature (PMID: 18489713). Use in ICC/IF reported in scientific literature (PMID: 21427217)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (100%), Bat (88%), Equine (88%), Rabbit (88%). Mouse reactivity reported in (PMID: 23273253). Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPER/GPR30 Antibody - BSA Free

• CEPR
• CEPRG-protein coupled receptor 30
• Chemoattractant receptor-like 2
• chemokine receptor-like 2
• CMKRL2
• CMKRL2MGC99678
• constitutively expressed peptide-like receptor
• DRY12
• DRY12IL8-related receptor DRY12
• FEG-1
• FEG-1mER
• Flow-induced endothelial G-protein coupled receptor 1
• G protein-coupled estrogen receptor 1
• G protein-coupled receptor 30
• GPCR-BR
• GPER
• GPR30
• GPR30GPCR-Br
• G-protein coupled estrogen receptor 1
• heptahelix receptor
• LERGU
• LERGU2
• leucine rich protein in GPR30 3'UTR
• LyGPR
• Lymphocyte-derived G-protein coupled receptor
• Membrane estrogen receptor

Background

GPR30 is a transmembrane intracellular estrogen receptor (Revankar et al. 2005). Progestin inhibits growth in breast cancer cells through GPR30. GPR30 has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. ESTs have been isolated from B-cell/lung/testis, brain, ganglion, heart/melanocyte/uterus, kidney, tonsil, liver/spleen, placenta, and prostate libraries. Ligands: Estrogen (Revankar et al. 2005). Caution: Rat GPR30 is also known as GPR41 and HIA-R.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GPER/GPR30 Antibody (NLS4271)(19)

Publications using NLS4271

• Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP. Norepinephrine Regulation of Adrenergic Receptor Expression, 5' AMP-Activated Protein Kinase Activity, and Glycogen Metabolism and Mass in Male Versus Female Hypothalamic Primary Astrocyte Cultures ASN Neuro 2020-11-12 [PMID: 33176438]
• Querido E, Sfeir A, Chartrand P ALKBH5 inhibited cell proliferation and sensitized bladder cancer cells to cisplatin by m6A-CK2 alpha mediated glycolysis STAR Protoc 2020-10-01 [PMID: 33111129] (IF/IHC, Human)
• Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP Sex differences in glucoprivic regulation of glycogen metabolism in hypothalamic primary astrocyte cultures: Role of estrogen receptor signaling Mol. Cell. Endocrinol. 2020-08-24 [PMID: 32853745] (WB, Rat)
• Briski KP, Ali MH, Napit PR Sex-Specific Acclimation of A2 Noradrenergic Neuron Dopamine-Beta-Hydroxylase and Estrogen Receptor Variant Protein and 5'-AMP-Activated Protein Kinase Reactivity to Recurring Hypoglycemia in Rat J. Chem. Neuroanat. 2020-06-26 [PMID: 32599255] (WB, Rat)
  
  Details:
  Sprague-Dawley rats
• Alshamrani AA, Bheemanapally K, Ibrahim MMH, Briski KP Impact of caudal hindbrain glycogen metabolism on A2 noradrenergic neuron AMPK activation and ventromedial hypothalamic nucleus norepinephrine activity and glucoregulatory neurotransmitter marker protein expression Neuropeptides 2020-05-16 [PMID: 32451071] (WB, Rat)
• Ibrahim MMH, Bheemanapally K, Sylvester PW, Briski KP Sex-specific estrogen regulation of hypothalamic astrocyte estrogen receptor expression and glycogen metabolism in rats Mol. Cell. Endocrinol. 2020-01-11 [PMID: 31931041] (WB, Rat)
• Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP Sex-Dimorphic Estrogen Receptor Regulation of Ventromedial Hypothalamic Nucleus Glucoregulatory Neuron Adrenergic Receptor Expression in Hypoglycemic Male and Female Rats Brain Res. 2019-06-29 [PMID: 31265816] (WB, Rat)
• Hasan Mahmood ASM, Mandal SK, Bheemanapally K et al. Norepinephrine control of ventromedial hypothalamic nucleus glucoregulatory neurotransmitter expression in the female rat: Role of monocarboxylate transporter function. Mol. Cell. Neurosci. 2019-01-17 [PMID: 30660767] (WB, Rat)
• Ghaffari S, Naderi Nabi F et al. Estrogen Inhibits LDL (Low-Density Lipoprotein) Transcytosis by Human Coronary Artery Endothelial Cells via GPER (G-Protein-Coupled Estrogen Receptor) and SR-BI (Scavenger Receptor Class B Type 1). Arterioscler Thromb Vasc Biol 2018-01-10 [PMID: 30354216] (WB, Human)
• Tica AA, Tica OS, Georgescu CV et al. GPER and ERalpha expression in abnormal endometrial proliferations. Rom J Morphol Embryol. 2016-08-12 [PMID: 27516013] (IF/IHC, Human)
• Jala VR, Radde BN, Haribabu B, Klinge CM. Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer. BMC Cancer 2012-12-28 [PMID: 23273253] (WB, IHC-P, Mouse, Human)
• Li Q, Sullivan NR, McAllister CE, Van de Kar LD, Muma NA. Estradiol accelerates the effects of fluoxetine on serotonin 1A receptor signaling Psychoneuroendocrinology 2012-12-04 [PMID: 23219224] (WB, Rat)
• McAllister CE, Creech RD, Kimball PA, Muma NA, Li Q. GPR30 is necessary for estradiol-induced desensitization of 5-HT(1A) receptor signaling in the paraventricular nucleus of the rat hypothalamus. Psychoneuroendocrinology. 2012-01-18 [PMID: 22265196] (WB, Rat)
• Maiti K, Paul JW, Read M et al. G-1-activated membrane estrogen receptors mediate increased contractility of the human myometrium Endocrinology 2011-06-01 [PMID: 21427217] (ICC/IF, WB, IF/IHC, Human)
• Jeng YJ, Kochukov M, Watson CS Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells. Environ Health. 2010-10-15 [PMID: 20950447] (ELISA, Rat)
• Park II, Zhang Q, Liu V et al. 17{beta}-estradiol at low concentrations acts through distinct pathways in normal versus BPH-derived prostate stromal cells. Endocrinology :en.2008-1591. 2009-01-01 [PMID: 19608654]
• Alyea RA, Watson CS. Nongenomic mechanisms of physiological estrogen-mediated dopamine efflux. BMC Neurosci. 2009-06-16 [PMID: 19531209] (ELISA, IP, Rat)
• Alyea RA, Watson CS. Differential regulation of dopamine transporter function and location by low concentrations of environmental estrogens and 17beta-estradiol. Environ Health Perspect. 2009-05-01 [PMID: 19479021] (WB, Human)
• Alyea RA, Laurence SE, Kim SH et al. The roles of membrane estrogen receptor subtypes in modulating dopamine transporters in PC-12 cells. J Neurochem. 2008-08-01 [PMID: 18489713] (WB, ELISA, Rat)

Review for GPER/GPR30 Antibody (NLS4271) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 01/24/2013

Summary

• Application: Western Blot
• Sample Tested: rat hypothalamus
• Species: Rat
• Lot: 8967

FAQs for GPER/GPR30 Antibody (NLS4271). (Showing 1 - 1 of 1 FAQs).

1. What is the MW band size for GPER with your GPR30 Antibody (NLS4271)?
   • The expected molecular weight of GPR30 is 42.3, however, because this protein has at least 3 different glycosylation sites, you should not be surprised if you see a band at higher position in your samples.

Bioinformatics

• Gene Symbol: GPER1
• Entrez:
  • 2852(Human)
• Uniprot:
  • Q99527()