GRB2 Antibody (SR1396) - BSA Free

Western Blot: GRB2 Antibody (SR1396) [NBP3-21851] - Western blot analysis using NBP3-21851 in HEK293 cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: SR1396
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GRB2 Antibody (SR1396) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human GRB2 (Uniprot #: P62993)
• Specificity: Detects endogenous levels of total GRB2
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GRB2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GRB2 Antibody (SR1396) - BSA Free

• abundant SRC homology
• Adapter protein GRB2
• ASH
• EGFRBP-GRB2
• epidermal growth factor receptor-binding protein GRB2
• GRB2
• Grb3-3
• growth factor receptor-bound protein 2
• growth factor receptor-bound protein 3
• HT027
• MST084
• MSTP084
• NCKAP2
• Protein Ash
• SH2/SH3 adapter GRB2

Background

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRB2