hnRNP K Antibody (N10 P2D3*G2) - BSA Free

Western Blot: hnRNP K Antibody (N10 P2D3G2) [NBP2-50424] - Detection of hnRNP K isoform 1, a strong band at 51 kDa is observed.

Immunohistochemistry: hnRNP K Antibody (N10 P2D3G2) [NBP2-50424] - Staining of formalin-fixed, paraffin-embedded tissue sections. Strong nuclear immunostaining was observed in normal skin (A). Nuclear and cytoplasmic immunostaining was observed in breast carcinoma (B). This is in agreement with existing literature as hnRNPK shows aberrant localisation in tumours.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: N10 P2D3*G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

hnRNP K Antibody (N10 P2D3*G2) - BSA Free Summary

• Immunogen: Ovalbumin-conjugated synthetic peptide SVKQYSGKFF (C-terminal sequence)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Positive control(s): IHC - formalin-fixed, paraffin-embedded colorectal cancer sections; WB - Jurkat cell lysate.

Reactivity Notes

Immunogen peptide sequence is common to mouse, rat, rabbit, chicken, sheep, dog, pig, horse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for hnRNP K Antibody (N10 P2D3*G2) - BSA Free

• dC-stretch binding protein
• FLJ41122
• heterogeneous nuclear ribonucleoprotein K
• hnRNP K
• transformation upregulated nuclear protein
• Transformation up-regulated nuclear protein
• TUNPHNRPKCSBP

Background

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPK