HO-1/HMOX1/HSP32 Antibody - BSA Free

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Mouse liver. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Analysis of HO-1 in HEK293 cell lysate using anti-HO-1 antibody. WB Image submitted by a verified customer review.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:1500.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Nrf2-pathway activation by hybrids: nuclear translocation and targets induction. Protein levels by Western blot. Anti-actin was used as protein loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32047434/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Human renal carcinoma . Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

ELISA: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - ELISA detection of recombinant full-length Heme Oxygenase 1 protein using as capture antibody at concentration of 5 ug/mL and as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - HO-1/HMOX1/HSP32 antibody detects HO-1/HMOX1/HSP32 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.HO-1/HMOX1/HSP32 stained by HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HO-1/HMOX1/HSP32 antibody (NBP1-31341) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HO-1/HMOX1/HSP32 Antibody [NBP1-31341] - Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ELISA, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HO-1/HMOX1/HSP32 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HO-1/HMOX1/HSP32. The exact sequence is proprietary.
• Localization: Microsome, Endoplasmic reticulum
• Marker: Oxidative Stress Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMOX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Sandwich ELISA Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (85%), Porcine (82%), Bovine (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for HO-1/HMOX1/HSP32 Antibody - BSA Free

• bK286B10
• EC 1.14.99.3
• heat shock protein, 32-kD
• heme oxygenase (decycling) 1
• HMOX1
• HO
• HO1
• HO-1
• HO-1heme oxygenase 1
• HSP32

Background

Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HO-1/HMOX1/HSP32 Antibody (NBP1-31341)(12)

Publications using NBP1-31341

• Kovacevic S, Ivanov M, Zivotic M Et al. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats Antioxidants (Basel) 2021-08-27 [PMID: 34439411] (IHC-P)
  
  Details:
  This citation used the HRP format of this antibody.
• Jing M, Cen Y, Gao F Et Al. Nephroprotective Effects of Tetramethylpyrazine Nitrone TBN in Diabetic Kidney Disease Frontiers in pharmacology 2021-06-24 [PMID: 34248629] (WB)
• Wen J, Li S, Zheng C et al. Tetramethylpyrazine nitrone improves motor dysfunction and pathological manifestations by activating the PGC-1 alpha/Nrf2/HO-1 pathway in ALS mice Neuropharmacology 2020-11-01 [PMID: 33152451] (WB, Mouse)
• Serafini M, Catanzaro M, Fagiani F et al. Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids Front. Pharmacol. 2020-01-28 [PMID: 32047434] (WB, Human)
• Rosini M, Simoni E, Caporaso R et al. Merging memantine and ferulic acid to probe connections between NMDA receptors, oxidative stress and amyloid-beta peptide in Alzheimer's disease Eur J Med Chem 2019-07-05 [PMID: 31301562] (Human)
• Toy R, Pradhan P, Ramesh V et al. Selenium Deficiency Is Associated with Pro-longevity Mechanisms Cell Rep 2019-05-28 [PMID: 31141699] (WB, Mouse)
• Mathew G, Sharma A, Pickering RJ et al. A novel synthetic small molecule DMFO targets Nrf2 in modulating proinflammatory/antioxidant mediators to ameliorate inflammation. Free Radic. Res. 2018-11-13 [PMID: 30422019] (ELISA, Mouse)
• Moon Su-Jin, Kim Eun-Kyung, Jhun Joo Yeon et al. The active metabolite of leflunomide, A77 1726, attenuates inflammatory arthritis in mice with spontaneous arthritis via induction of heme oxygenase-1. Journal of Translational Medicine 2017-01-01 [PMID: 28193225] (ICC/IF, WB, Mouse)
• Bakkar N, Kousari A, Kovalik T et al. RBM45 Modulates the Antioxidant Response in Amyotrophic Lateral Sclerosis through Interactions with KEAP1. Mol Cell Biol 2015-07-01 [PMID: 25939382] (WB, Human)
• Pambianchi E, Hagenberg Z, Pecorelli A Et al. Alaskan Bog Blueberry (Vaccinium uliginosum) Extract as an Innovative Topical Approach to Prevent UV-Induced Skin Damage Cosmetics 2021-11-27 (WB, Human)
• Vogtmann R, Burk LV, Serdar M et al. Systemic Maternal Human sFLT1 Overexpression Leads to an Impaired Foetal Brain Development of Growth-Restricted Foetuses upon Experimental Preeclampsia Oxidative medicine and cellular longevity 2022-06-02 [PMID: 35693702] (WB, Mouse)
• Fagiani F, Catanzaro M, Buoso E Targeting Cytokine Release Through the Differential Modulation of Nrf2 and NF-kB Pathways by Electrophilic/Non-Electrophilic Compounds Front Pharmacol 2020-09-14 [PMID: 32922294]

Review for HO-1/HMOX1/HSP32 Antibody (NBP1-31341) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/20/2015

Summary

• Application: Western Blot
• Sample Tested: HEK-293 whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: HMOX1