HSP70/HSPA1A Antibody (SA0379)

Western Blot: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Western blot analysis of HSP70/HSPA1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Staining Hsp70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-HSP70/HSPA1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% B

Flow Cytometry: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Analysis of Hsp70 on different cell lysates using anti-Hsp70 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: HCT116

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Staining Hsp70 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Analysis of paraffin-embedded mouse brain tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Analysis of paraffin-embedded mouse prostate tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Analysis of paraffin-embedded mouse testis tissue using anti-Hsp70 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HSP70/HSPA1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocke

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-HSP70/HSPA1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BS

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-HSP70/HSPA1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (SA0379) [NBP2-67287] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-HSP70/HSPA1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked

Product Discontinued

HSP70/HSPA1A Antibody (SA0379) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human HSP70/HSPA1A aa 403-641 / 641. (SwissProt: P0DMV9 Human; SwissProt: P0DMV8 Human; SwissProt: P17879 Mouse; SwissProt: Q61696 Mouse; SwissProt: Q07439 Rat)
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPA1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSP70/HSPA1A Antibody (SA0379)

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA1A