IL-1 RAcP/IL-1 R3 Antibody - BSA Free

Western Blot: IL-1 RAcP/IL-1 R3 Antibody [NBP1-76924] - Analysis of IL-1RAcP expression in K562 cell lysate with IL-1RAcP antibody at 1 ug/ml.

Western Blot: IL-1 RAcP/IL-1 R3 Antibody [NBP1-76924] - HeLa whole cell lysate with IL1RAP antibody at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: IL-1 RAcP/IL-1 R3 Antibody - BSA Free [NBP1-76924] - Immunocytochemistry of IL-1 RAcP/IL-1 R3 in HeLa cells with IL-1 RAcP/IL-1 R3 antibody at 5 ug/mL.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-1 RAcP/IL-1 R3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 22 amino acids near the carboxy terminus of human IL-1RAcP. The immunogen is located within the last 50 amino acids of IL-1RAcP. Amino Acid Squence: KKSPRRSSSDEQGLSYSSLKNV
• Specificity: It has no cross activity to other member in the IL-1 receptor family.
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL1RAP
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-1 RAcP/IL-1 R3 Peptide (NBP1-76924PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-1 RAcP/IL-1 R3 Antibody - BSA Free

• C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3
• FLJ37788
• IL-1 R3
• IL-1 RAcP
• IL-1 receptor accessory protein
• IL-1R3
• IL-1R-3
• IL1RAcP
• IL-1RAcP
• IL1RAP
• interleukin 1 receptor accessory protein
• interleukin-1 receptor accessory protein beta
• interleukin-1 receptor accessory protein

Background

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kappaB inducing kinae (NIK) and of NF-kappaB, recruites to the IL-1R complex through IL-1 RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL1RAP
• Uniprot:
  • AF029213(Human)