Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 by WB of HeLa treated with IFN-gamma (+) or mock treated (-), HEK293T, HDLM-2, Hep-G2, IDO2 over-expressing HEK, untransfected HEK, and IDO1 over-expresssing HEK lysate. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Vinculin.

Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 (red) in FFPE skin carcinoma by IHC-IF. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] NBP2-76404. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE tonsil by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE A-549 cells untreated (left) or treated with hIFN-gamma (right) by ICC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) [NBP2-76404] - Detection of human IDO1 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-IDO1 recombinant monoclonal [BLR031F] (NBP2-76404). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: BLR031F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein representing Human IDO1
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IDO1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 -1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for Indoleamine 2,3-dioxygenase/IDO Antibody (BLR031F)

• 3dioxygenase
• EC 1.13.11.52
• IDO
• IDO1
• IDOIDO-1
• INDO
• INDOindole 2,3-dioxygenase
• Indoleamine 2
• indoleamine 2,3-dioxygenase 1
• Indoleamine 2,3-dioxygenase
• indoleamine-pyrrole 2,3 dioxygenase
• Indoleamine-pyrrole 2,3-dioxygenase

Background

Indoleamine 2,3-dioxygenase (IDO1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. IDO1 acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDO1