KDM2A/FBXL11 Antibody - BSA Free

Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KCM2A/FBXL11 Antibody (NBP1-78305) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry: KDM2A/FBXL11 Antibody [NBP1-78305] - IHC analysis of KDM2A / FBXL11 in human kidney cancer xenograft using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody [NBP1-78305] - Human astrocytoma cell line lentivirally transduced and expressing KDM2A. Cells were fixed with 4% paraformaldehyde and stained with anti-KDM2A, followed by Alexa Fluor 488-anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KDM2A/FBXL11 Antibody (NBP1-78305) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.2 mg/ml

KDM2A/FBXL11 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an N-terminal portion of the human KDM2A/FBXL11 protein (between residues 350-500) [UniProt Q9Y2K7]
• Localization: Nucleus, nucleoplasm. Note: Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
  • Western Blot reported by customer review
• Application Notes: This KDM2A/FBXL11 antibody is useful for Immunocytochemistry/Immunofluorescence where nuclear staining is observed in HeLa cells and IHC-paraffin embedded sections where nuclear staining is seen in human kidney cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KDM2A/FBXL11 Antibody - BSA Free

• [Histone-H3]-lysine-36 demethylase 1A
• CXXC8
• CXXC8F-box protein FBL7
• CXXC-type zinc finger protein 8
• EC 1.14.11.27
• FBL11
• FBL11DKFZp434M1735
• FBL7
• FBL7F-box protein Lilina
• F-box and leucine-rich repeat protein 11DKFZP434M1735
• F-box protein FBL11
• F-box protein FBL7
• FBXL11
• FBXL11FLJ46431
• FLJ00115
• JHDM1A
• JHDM1AF-box/LRR-repeat protein 11
• JmjC domain-containing histone demethylation protein 1A
• jumonji C domain-containing histone demethylase 1A
• KDM2A
• KDM2A/FBXL11
• KIAA1004
• KIAA1004EC 1.14.11.27
• LILINA
• lysine (K)-specific demethylase 2A
• lysine-specific demethylase 2A
• SF-KDM2A

Background

KDM2A / FBXL11 (also known as JHDM1A, lysine specific demethylase 1A) is a F-box family member and JmjC-domain containing histone demethylase that specifically demethylates Lysine 36 of H3 (H3 K36). KDM2A / FBXL11 is involved in epigenetic silencing and required to maintain the heterochromatic state, as well as sustaining centromeric integrity and genomic stability during mitosis (PMID 19001877). Under conditions of starvation, FBXL11's ability to repress RNA transcription has been shown to be reduced (PMID 20379134). In addition, KDM2A / FBXL11 is also a key inhibitory regulator of NF kappa B activity. This regulation occurs when K218 and K221 of the p65 subunit are methylated. The overexpression of KDM2A / FBXL11 can decrease the viability of specific cancer cell lines, while downregulation was shown to have the opposite effect (PMID 20080798). KDM2A / FBXL11 shows further regulatory involvement at CpG islands. Nonmethylated CpG islands preferentially bind KDM2A / FBXL11 using a zinc finger domain, in which the binding is blocked when CpG DNA is in its methlylated form (PMID 20417597).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for KDM2A/FBXL11 Antibody (NBP1-78305) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/31/2019

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human
• Lot: A-1

reviewed by: Verified Customer ICC Human 12/31/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: AGS human gastric adenocarcinoma cell line,brain tumor cell line
• Species: Human
• Lot: A-1

reviewed by: Verified Customer WB Human 05/18/2016

Summary

• Application: Western Blot
• Sample Tested: Human lung cancer cell lines whole cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: KDM2A
• Entrez:
  • 22992(Human)
• Uniprot:
  • Q9Y2K7(Human)