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Images
Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.
Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
Immunohistochemistry: KDM2A/FBXL11 Antibody [NBP1-78305] - IHC analysis of KDM2A / FBXL11 in human kidney cancer xenograft using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: KDM2A/FBXL11 Antibody [NBP1-78305] - Human astrocytoma cell line lentivirally transduced and expressing KDM2A. Cells were fixed with 4% paraformaldehyde and stained with ...read more
Immunocytochemistry/ Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
A synthetic peptide made to an N-terminal portion of the human KDM2A/FBXL11 protein (between residues 350-500) [UniProt Q9Y2K7]
Localization
Nucleus, nucleoplasm. Note: Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
This KDM2A/FBXL11 antibody is useful for Immunocytochemistry/Immunofluorescence where nuclear staining is observed in HeLa cells and IHC-paraffin embedded sections where nuclear staining is seen in human kidney cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 3 Reviews rated 4.7 using NBP1-78305 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified
Alternate Names for KDM2A/FBXL11 Antibody - BSA Free
[Histone-H3]-lysine-36 demethylase 1A
CXXC8
CXXC8F-box protein FBL7
CXXC-type zinc finger protein 8
EC 1.14.11.27
FBL11
FBL11DKFZp434M1735
FBL7
FBL7F-box protein Lilina
F-box and leucine-rich repeat protein 11DKFZP434M1735
F-box protein FBL11
F-box protein FBL7
FBXL11
FBXL11FLJ46431
FLJ00115
JHDM1A
JHDM1AF-box/LRR-repeat protein 11
JmjC domain-containing histone demethylation protein 1A
jumonji C domain-containing histone demethylase 1A
KDM2A
KDM2A/FBXL11
KIAA1004
KIAA1004EC 1.14.11.27
LILINA
lysine (K)-specific demethylase 2A
lysine-specific demethylase 2A
SF-KDM2A
Background
KDM2A / FBXL11 (also known as JHDM1A, lysine specific demethylase 1A) is a F-box family member and JmjC-domain containing histone demethylase that specifically demethylates Lysine 36 of H3 (H3 K36). KDM2A / FBXL11 is involved in epigenetic silencing and required to maintain the heterochromatic state, as well as sustaining centromeric integrity and genomic stability during mitosis (PMID 19001877). Under conditions of starvation, FBXL11's ability to repress RNA transcription has been shown to be reduced (PMID 20379134). In addition, KDM2A / FBXL11 is also a key inhibitory regulator of NF kappa B activity. This regulation occurs when K218 and K221 of the p65 subunit are methylated. The overexpression of KDM2A / FBXL11 can decrease the viability of specific cancer cell lines, while downregulation was shown to have the opposite effect (PMID 20080798). KDM2A / FBXL11 shows further regulatory involvement at CpG islands. Nonmethylated CpG islands preferentially bind KDM2A / FBXL11 using a zinc finger domain, in which the binding is blocked when CpG DNA is in its methlylated form (PMID 20417597).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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![Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDM2A-FBXL11-Antibody-Western-Blot-NBP1-78305-img0006.jpg "Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KCM2A/FBXL11 Antibody (NBP1-78305) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/KDM2A-FBXL11-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-78305-img0008.jpg "Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KCM2A/FBXL11 Antibody (NBP1-78305) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: KDM2A/FBXL11 Antibody [NBP1-78305] - IHC analysis of KDM2A / FBXL11 in human kidney cancer xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/KDM2A-FBXL11-Antibody-Immunohistochemistry-NBP1-78305-img0004.jpg "Immunohistochemistry: KDM2A/FBXL11 Antibody [NBP1-78305] - IHC analysis of KDM2A / FBXL11 in human kidney cancer xenograft using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody [NBP1-78305] - Human astrocytoma cell line lentivirally transduced and expressing KDM2A. Cells were fixed with 4% paraformaldehyde and stained with anti-KDM2A, followed by Alexa Fluor 488-anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KDM2A-FBXL11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78305-img0005.jpg "Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody [NBP1-78305] - Human astrocytoma cell line lentivirally transduced and expressing KDM2A. Cells were fixed with 4% paraformaldehyde and stained with anti-KDM2A, followed by Alexa Fluor 488-anti-rabbit secondary antibody. DAPI staining shows nuclei. ICC/IF image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KDM2A/FBXL11 Antibody (NBP1-78305) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/KDM2A-FBXL11-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-78305-img0007.jpg "Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Caco-2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KDM2A/FBXL11 Antibody (NBP1-78305) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
