PSD-95 Antibody (SR38-09)

Western Blot: PSD-95 Antibody (SR38-09) [NBP2-67322] - Analysis of PSD95 on different lysates using anti-PSD95 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Human brain

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (SR38-09) [NBP2-67322] - Staining PSD95 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PSD-95 Antibody (SR38-09) [NBP2-67322] - Analysis of rat cerebellum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.

Flow Cytometry: PSD-95 Antibody (SR38-09) [NBP2-67322] - Analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (SR38-09) [NBP2-67322] - Staining PSD95 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PSD-95 Antibody (SR38-09) [NBP2-67322] - Analysis of mouse cerebullum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PSD-95 Antibody (SR38-09) [NBP2-67322] - Analysis of rat brain tissue using anti-PSD95 antibody. Counter stained with hematoxylin.

Product Discontinued

PSD-95 Antibody (SR38-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human PSD-95 aa 1-40. (SwissProt: P78352 Human; SwissProt: Q62108 Mouse; SwissProt: P31016 Rat)
• Localization: Cell membrane, Cell junction
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DLG4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PSD-95 Antibody (SR38-09)

• discs, large homolog 4 (Drosophila)
• disks large homolog 4
• DLG4
• FLJ97752
• FLJ98574
• Postsynaptic density protein 95
• post-synaptic density protein 95
• PSD95
• PSD-95
• PSD95SAP90SAP-90discs large homolog 4
• SAP90
• Synapse-associated protein 90
• Tax interaction protein 15

Background

Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG4