PSD93 [p Tyr340] Antibody - BSA Free

Western Blot: PSD93 [p Tyr340] Antibody [NBP3-38644] - Western blot analysis of various lysates using PSD93 Rabbit pAb at 1:1000 dilution. 293T cells were treated by ATP(5 mM) at 30C for 1 hour. NIH/3T3 cells were treated by ATP(5 mM) at 30C for 1 hour. C6 cells were treated by ATP(5 mM) at 30C for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSD93 [p Tyr340] Antibody - BSA Free Summary

• Immunogen: A synthetic phosphorylated peptide around Y340 of human PSD93 (NP_001355.2).
  
  Sequence:
  DDYTR
• Modification: p Tyr340
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLG2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 98 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for PSD93 [p Tyr340] Antibody - BSA Free

• Chapsyn-110
• discs, large homolog 2 (Drosophila)
• discs, large homolog 2, chapsyn-110 (Drosophila)
• discs, large homolog 2, chapsyn-110
• disks large homolog 2
• DKFZp781D1854
• DKFZp781E0954
• FLJ37266
• MGC131811
• Postsynaptic density protein PSD-93
• PSD-93
• PSD93,110kDa

Background

Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG2