RALBP1 Antibody (OTI11B2)

Western Blot: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.

Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded prostate tissue using anti-RALBP1 mouse monoclonal antibody.

Flow Cytometry: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Analysis of Jurkat cells, using anti-RALBP1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: RALBP1 Antibody (OTI11B2) [NBP2-02293] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.

Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded breast tissue using anti-RALBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-RALBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-RALBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded endometrium tissue using anti-RALBP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded Ovary tissue using anti-RALBP1 mouse monoclonal antibody.

Immunoprecipitation: RALBP1 Antibody (OTI11B2) [NBP2-02293] - (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RALBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: OTI11B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.84 mg/ml

RALBP1 Antibody (OTI11B2) Summary

• Immunogen: Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RALBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 4ug/ml
  • Western Blot 1:2000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.84 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RALBP1 Antibody (OTI11B2)

• Dinitrophenyl S-glutathione ATPase
• ralA binding protein 1
• ralA-binding protein 1,76 kDa Ral-interacting protein
• RalBP1
• Ral-interacting protein 1
• RIP
• RIP1
• RLIP1
• RLIP76DNP-SG ATPase

Background

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for RALBP1 Antibody (NBP2-02293). (Showing 1 - 1 of 1 FAQ).

1. Our customer would like to know if NBP2-02293 will recognize phospho-RALBP1 or not.
   • NBP2-02293 was raised against full length human recombinant protein of human RALBP1. It was not raised against the phosphorylated protein and we cannot therefore guarantee that it will recognize the phosphorylated version. We have tested it against lysates however have not determined the phosphorylation status.

Bioinformatics

• Gene Symbol: RALBP1
• Entrez:
  • 866(Human)