VprBP Antibody

Western Blot: VprBP Antibody [NBP2-27203] - analysis of VPRBP using VPRBP antibody. Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of VPRBP antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Flow Cytometry: VprBP Antibody [NBP2-27203] - Intracellular analysis of VPRBP in human HeLa and mouse RAW cells using VPRBP antibody at 0.2 ug/10^6 cells. Red represents cells with VPRBP antibody, green represents isotype control antibody.

• Reactivity: Hu, Mu, Rt, Po, Ch, ChHa, Eq, Fe, Gp, Ma-Op, Pm, Rb, Bv, Pm-Or
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VprBP Antibody Summary

• Immunogen: A portion of amino acids 935-985 from human VPRBP was used as the immunogen for this antibody.
• Predicted Species: Orangutan (100%), Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VPRBP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.2ug/10^6cells
  • Western Blot 2-4ug/ml~

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in White-cheeked Gibbon, and 88% homolgous in western clawed Frog.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for VprBP Antibody

• DCAF1DDB1 and CUL4 associated factor 1
• DDB1- and CUL4-associated factor 1
• HIV-1 Vpr-binding protein
• KIAA0800protein VPRBP
• MGC102804
• RIP
• Vpr (HIV-1) binding protein
• Vpr-binding protein
• VprBP
• Vpr-interacting protein

Background

VPRBP binds and bridges the CUL4 and DDB1 ubiquitin ligase complex, binding the DNA damage binding protein DDB1 and CUL4. VPRBP is the acronym for the human immunodeficiency virus type-1 Vpr binding protein (1-3). CUL4 is a cullin family memember. Cullins are ubiquitin ligases that are cable of ubiquitinylating a wide range of protein substrates (4). All ubiquitin ligases are important as the modification of proteins by ubiquitin addition modifies or activates functionality (4, 5). VPRBP is critical for normal cell cycle progression through S phase and allows proper cell proliferation and development. VPRBP appears to serve in an epigenetic role through regulation of histone methylation (5). VPRBP antibody provides a reagent for exploration of ubiqitin ligase activities in cell cycle and other cell pathways responsible for normal cell growth and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VPRBP
• Uniprot:
  • Q9Y4B6(Human)