ROBLD3 Antibody

Immunocytochemistry/Immunofluorescence: ROBLD3 Antibody [NBP1-71687] - ROBLD3 Antibody was tested at 1:100 in NIH/3T3 cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).

Immunohistochemistry: ROBLD3 Antibody [NBP1-71687] - Analysis of ROBLD3 in human renal cell carcinoma.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ROBLD3 Antibody Summary

• Immunogen: A genomic peptide made to an N-terminal region of the human ROBLD3 protein (within residues 1-125). [Swiss-Prot Q9Y2Q5]
• Localization: Late endosome membrane and Lysosome membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAMTOR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
• Application Notes: This ROBLD3 antibody is useful for IHC-P and ICC/IF. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.1% BSA, and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for ROBLD3 Antibody

• ENDAP
• Endosomal adaptor protein p14
• HSPC003
• Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2
• late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
• Late endosomal/lysosomal Mp1-interacting protein
• MAPBPIPRoadblock domain-containing protein 3
• MAPKSP1 adaptor protein
• MAPKSP1AP
• mitogen-activated protein-binding protein-interacting protein (MAPBPIP)
• Mitogen-activated protein-binding protein-interacting protein
• p14Ragulator2
• roadblock domain containing 3
• ROBLD3

Background

ROBDL3/p14 is a major component of the Ragulator complex, which interacts with Rag GTPases and partially activates mTORC1 kinase. This interaction ultimately leads to growth, with influence of growth factors, energy levels, and amino acids. ROBDL3 is intrinsically related to lysosomal function, and endosomal transport regulation. It binds Nbr1 and the two are associated with protein turnover regulation, while it is required to localize MEK1 partner protein to the endosome. Endosomal localization of ROBLD3 can be affected by the interaction of p18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMTOR2
• Entrez:
  • 28956(Human)
  • 56692(Mouse)
• Uniprot:
  • Q9Y2Q5(Human)
  • O88653(Mouse)