TAK1 Antibody

Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.

Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.

Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: TAK1 Antibody [NB100-56363] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of TAK1 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NB100-56363] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TAK1 Antibody NB100-56363 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TAK1 Antibody Summary

• Immunogen: This polyclonal antibody was raised against amino acids 19-35 and 564-579 of human TAK1 (accession number BAA25025).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-5 ug/ml
  • Immunohistochemistry 1:200. Use reported in scientific literature (PMID 30786091)
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1-2 ug/ml
• Application Notes: In NIH 3T3, an approx. 70 kDa band is observed.
• Control:

  NIH 3T3 Whole Cell Lysate

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Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for TAK1 Antibody

• EC 2.7.11
• EC 2.7.11.25
• MAP3K7
• MEKK7
• mitogen-activated protein kinase kinase kinase 7
• TAK1
• TAK1/MAP3K7
• TAK1TGF1a
• TGF1a
• TGF-beta activated kinase 1
• TGF-beta-activated kinase 1
• Transforming growth factor-beta-activated kinase 1

Background

TAK1 was shown to participate in regulation of transcription by transforming growth factor-beta (TGF-beta). TAK1 is stimulated in response to TGF-beta and bone morphogenetic protein. These results suggest that TAK1 functions as a mediator in the signaling pathway of TGF-beta superfamily members. TAB1 and TAB2 are TAK1 binding proteins that may function as activators of the TAK1 (TGF-b activated kinase 1) MAPKKK in TGF-b signal transduction. TAB1-induced TAK1 activation promoted the dissociation of active forms of IKKa and IKK b from active TAK1, whereas the IKK mutants remained to interact with active TAK1. TNF-a activated endogenous TAK1, and the kinase-negative TAK1 acted as a dominant negative inhibitor against TNF-a-induced NF-kB activation. TAK1 was suggested to act as a regulatory kinase of IKKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TAK1 Antibody (NB100-56363)(2)

Publications using NB100-56363

• Sung Hsieh HH, Agarwal S, Cholok DJ et al. Coordinating Tissue Regeneration through TGF-b Activated Kinase 1 (TAK1) In-activation and Re-activation Stem Cells 2019-02-20 [PMID: 30786091] (IF/IHC, Mouse)
• Liepelt A, Mossanen JC, Denecke B et al. Translation control of TAK1 mRNA by hnRNP K modulates LPS-induced macrophage activation. RNA 2014-04-21 [PMID: 24751651] (WB, Mouse)
  
  Details:
  RAW264.7 and bone marrow-derived macrophages: IF (Figs 4A, 6A), WB (Fig 4B).

Bioinformatics

• Gene Symbol: MAP3K7
• Entrez:
  • 6885(Human)
  • 26409(Mouse)
• Uniprot:
  • O43187()