Transglutaminase 1/TGM1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Analysis in human esophagus and liver tissues using NBP2-34062 antibody. Corresponding TGM1 RNA-seq data are presented for the same tissues.

Western Blot: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Analysis in human tonsil tissue.

Immunohistochemistry-Paraffin: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Staining of human esophagus shows high expression.

Immunohistochemistry-Paraffin: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Staining of human colon shows no positivity in glandular cells.

Immunohistochemistry-Paraffin: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Staining of human skin shows moderate membranous positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: Transglutaminase 1/TGM1 Antibody [NBP2-34062] - Staining of human tonsil shows moderate membranous positivity in sqamous epithelial cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Transglutaminase 1/TGM1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: MMDGPRSDVGRWGGNPLQPPTTPSPEPEPEPDGRSRRGGGRSFWARCCGCCSCRNAADDDWGPEPSDSRGRGSSSGTRRPGSRGSDSRRPVSRGSGVNAA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Transglutaminase 1/TGM1 Protein (NBP2-34062PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Transglutaminase 1/TGM1 Antibody - BSA Free

• Epidermal TGase
• ICR2
• KTG
• LI
• LI1
• LI1EC 2.3.2.13
• protein-glutamine gamma-glutamyltransferase K
• protein-glutamine-gamma-glutamyltransferase)
• TG(K)
• TGASE
• TGase-1
• TGK
• TGKTGase K
• TGM1
• transglutaminase 1 (K polypeptide epidermal type I
• Transglutaminase 1
• Transglutaminase K
• transglutaminase, keratinocyte
• transglutaminase-1

Background

Tissue transglutaminase is a calcium activated enzyme which catalyzes the cross linking of proteins and the conjugation of polyamines to proteins. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD). CD, a genetic, immunologically mediated small bowel enteropathy that causes malabsorption, is one of the more common disorders in Western countries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 1/TGM1 Antibody (NBP2-34062)(2)

Publications using NBP2-34062

• Parisi L, Mockenhaupt C, Rihs S et al. Consistent downregulation of the cleft lip/palate-associated genes IRF6 and GRHL3 in carcinomas Frontiers in oncology 2022-11-15 [PMID: 36457487] (ICC/IF, Human)
• Salameh S, Tissot N, Cache K et al. A perfusable vascularized full-thickness skin model for potential topical and systemic applications Biofabrication 2021-07-01 [PMID: 33910175]

Bioinformatics

• Gene Symbol: TGM1
• Uniprot:
  • P22735(Human)