TSFM Antibody

Western Blot: TSFM Antibody [NBP2-20746] - Sample (30 ug of whole cell lysate) A: Jurkat B: K562 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: TSFM Antibody [NBP2-20746] - Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: TSFM protein stained by TSFM antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: TSFM Antibody [NBP2-20746] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

• Reactivity: Hu, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TSFM Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TSFM. The exact sequence is proprietary.
• Localization: Mitochondrion
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TSFM
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%), Rat (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TSFM Antibody

• COXPD3
• EFTS
• EF-Ts
• EFTSMT
• EF-Tsmt
• mitochondrial elongation factor Ts
• mitochondrial
• Ts translation elongation factor, mitochondrial

Background

Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TSFM