UCH-L1/PGP9.5 Antibody

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NB100-1616] - Staining of epithelial cells in porcine distal colon. Nerve fibers immunoreactive for the neural marker PGP 9.5 (red) are in close proximity to both IgA-immunoreactive plasma cells (green) and epithelial cells immunoreactive for SC (blue) in the colonic crypts.

Immunohistochemistry: UCH-L1/PGP9.5 Antibody [NB100-1616] - PGP9.5 staining of epithelial cells in porcine distal colon. Nerve fibers immunoreactive for the neural marker PGP 9.5

Product Discontinued

UCH-L1/PGP9.5 Antibody Summary

• Immunogen: Synthetic peptide: ASSEDTLLKDAAKVCR, corresponding to amino acids 176-191 of Human PGP9.5.
• Localization: Cytoplasmic
• Marker: pan-Neuronal Marker
• Specificity: PGP9.5
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UCHL1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)
  UCH-L1/PGP9.5 Overexpression Lysate

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Horse (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for UCH-L1/PGP9.5 Antibody

• EC 3.4.19.12
• EC 6.-
• Neuron cytoplasmic protein 9.5
• PARK5
• PGP 9.5
• PGP9.5
• PGP9.5Uch-L1
• PGP95
• ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• ubiquitin carboxyl-terminal hydrolase isozyme L1
• ubiquitin C-terminal hydrolase
• Ubiquitin thioesterase L1
• UCHL1
• UCH-L1

Background

PGP9.5 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. It is present in neurons and in cells of the diffuse neuroendocrine system. PGP9.5 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. This enzyme is also known as UCH-L1. Because of its abundance in nerves, it has been widely used as a marker for peripheral nerve fibers

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for UCH-L1/PGP9.5 Antibody (NB100-1616). (Showing 1 - 2 of 2 FAQ).

1. Do you have any data for rat in IHC?
   • For product NB100-1616, rat is listed based on the homology it shares to the immunogen for this antibody. We therefore do not have any direct testing data of this product on rat tissue samples.
2. Can you provide the lot specific concentration for item NB100-1616 lot 401523?
   • Since the antibody is supplied as whole antisera and not affinity purified antibody,we do not supply a concentration, since it would be the total protein in the sera rather than the specific antibody.

Bioinformatics

• Gene Symbol: UCHL1
• Entrez:
  • 7345(Human)
  • 22223(Mouse)
  • 29545(Rat)
• Uniprot:
  • P09936(Human)