CARD11/CARMA1 Antibody - BSA Free

Western Blot: CARD11/CARMA1 Antibody [NBP1-77219] - Western blot analysis of CARD11 expression in mouse thymus cell lysate with anti-CARMA1 using antibody at 0.5 (lane A), 1 (lane B), and 2 ug /ml (lane C), respectively.

Immunohistochemistry: CARD11/CARMA1 Antibody - BSA Free [NBP1-77219] - Immunohistochemistry of CARD11/CARMA1 in mouse thymus with CARD11/CARMA1 antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: CARD11/CARMA1 Antibody - BSA Free [NBP1-77219] - Immunofluorescence of CARD11/CARMA1 in Mouse Spleen cells with CARD11/CARMA1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CARD11/CARMA1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1. The immunogen is located within the last 50 amino acids of CARMA1. Amino Acid Squence: EEQRKTIWVDEDQL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD11
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: A band at approximately 125 kDa can be detected in Western Blot.
• Control Peptide:

  CARD11/CARMA1 Peptide (NBP1-77219PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CARD11/CARMA1 Antibody - BSA Free

• BIMP3
• BIMP3card-maguk protein 1
• CARD11
• CARD-containing MAGUK protein 1
• Carma 1
• CARMA1
• CARMA1bcl10-interacting maguk protein 3
• caspase recruitment domain family, member 11
• caspase recruitment domain-containing protein 11
• MGC133069

Background

CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IkappaK complex, thereby regulating activation of the NF-kappaB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARD11
• Uniprot:
  • Q9BXL7(Human)