hnRNP K Antibody

Western Blot: hnRNP K Antibody [NBP2-24532] - analysis of hnRNPK in K562 cell lysate in the 1) absence and2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532] - Formalin-fixed, paraffin-embedded human adrenal tissue probed with hnRNPK antibody at 5 ug/ml.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP2-24532] - Formalin-fixed, paraffin-embedded human thymus probed with hnRNPK antibody at 5 ug/ml.

Simple Western: hnRNP K Antibody [NBP2-24532] - Simple Western lane view shows a specific band for hnRNP K in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Ma-Op, Pm, Pm, Eq, Pm
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

hnRNP K Antibody Summary

• Immunogen: A portion of amino acid 200 to 250 of human hnRNPK was used as immunogen for this antibody.
• Predicted Species: Monkey (100%), Equine (93%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HNRNPK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Simple Western 1:50
  • Western Blot 2-5 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 61 kDa.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for hnRNP K Antibody

• dC-stretch binding protein
• FLJ41122
• heterogeneous nuclear ribonucleoprotein K
• hnRNP K
• transformation upregulated nuclear protein
• Transformation up-regulated nuclear protein
• TUNPHNRPKCSBP

Background

The hnRNP-K family of acidic nuclear proteins has been identified using a monoclonal antibody that distinguishes between quiescent and proliferating human keratinocytes. The family, which is composed of four major proteins (hnRNPs-K A, B, C and D) and their modified forms, is present in similar overall levels in quiescent and proliferating normal keratinocytes although clear differences were observed in the levels of some of the individual variants (1). hnRNP-K is an ancient RNA/DNA-binding protein that is involved in multiple processes that compose gene expression. The pleiotropic action of K protein reflects its ability to interact with different classes of factors, interactions that are regulated by extracellular signals (2). hnRNP-K has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several reports implicate hnRNP K having a role in tumorigenesis, for instance hnRNP K increases transcription of the oncogene c-myc and hnRNP K expression is regulated by the p53/MDM 2 pathway (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPK