NLRP1/NALP1 Antibody

Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM+) in the GCL. Image collected and cropped by Citeab from the following publication (Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Front Mol Neurosci (2019) licensed under a CC-BY license.

Western Blot: NLRP1/NALP1 Antibody [NB100-56148] - Analysis of NLRP1/NALP1 in adult human tissue lysates using NLRP1 antibody at 1:2000. Full-length NLRP1 protein was detected at highest levels in kidney, brain and epidermis. A smaller band was detected in thymus, possibly representing a NLRP1/NALP1 breakdown product or alternatively spliced form. 293T/NALP1: positive control, 293T cells transiently transfected with full-length NLRP1.

Immunohistochemistry: NLRP1/NALP1 Antibody [NB100-56148] - The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naive and NT control retinas: NLRP3 (green) is only expressed in blood vessels (white arrowheads). Twenty four hours after OHT, NLRP3 colocalizes with NLRP1 in RGCs (yellow arrows) and to RBPMS- cells (white arrows). Blue arrowheads denote labeling in RGC axons and dendrites. Bar, 25 um on all panels. Image collected and cropped by Citeab from the following publication (Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury. Front Mol Neurosci (2019) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE normal human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) epidermis, (B) esophagus (C) uterine cervix, and (D) thymus. Hematoxylin-eosin counterstain. In A-C, NALP1 expression is associated with differentiation in stratified epithelial of the skin, esophagus, and cervix.

Immunohistochemistry-Paraffin: NLRP1/NALP1 Antibody [NB100-56148] - FFPE human tissue sections stained for NALP1 (CARD7/NAC) using NB100-56148 at 1:2000. (A) bone marrow and (C) brain glioma core from a brain cancer microarray. (B) and (D) are higher magnifications of (A) and (C) respectively. Hemaxotylin-eosin counterstain.

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] - Gene expression & cellular distribution of NLRP1, NLRP3 & Aim2 sensors in the retina. (A) RNAscope analysis of NLRP1 (green dots, yellow arrows) & NLRP3 (white dots, white arrows) transcript abundance in normotensive control (NT control) & experimental (OHT) retinas at 12 h postinjury. (B) Aim2 transcript (red dots) abundance in normotensive control & experimental retinas. Co-staining for the Muller glia marker glutamine synthetase (GlSyn, green) is added to indicate cellular expression of Aim2 in the GCL layer (yellow arrows) & INL (white arrows) in control & 12 h post-OHT retinas. Bar, 25 μm on all panels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody [NB100-56148] - OHT-induced upregulation of Aim2 inflammasome in the retina. (A) NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM+) in the GCL. (B) Immunohistochemistry for Aim2 protein (red) & Muller glia marker (GlSyn, green). Arrows show colocalization with Muller glia; no colocalization is detected with astrocytes (GFAP, white) in both control & 24 h post-OHT retinas. (C) NLRP3 labeling (green) in the inner retina of naïve & control eyes localized only to blood capillaries (white arrowhead) & astrocytes (yellow arrows), but not to RGCs (RBPMS cells). At 24 h post-injury the labeling in large cells in the GCL & INL (white arrows) are observed. (D) The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naïve & NT control retinas: NLRP3 (green) is only expressed in blood vessels (white arrowheads). Twenty four hours after OHT, NLRP3 colocalizes with NLRP1 in RGCs (yellow arrows) & to RBPMS- cells (white arrows). Blue arrowheads denote labeling in RGC axons & dendrites. Bar, 25 μm on all panels. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NLRP1/NALP1 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 161-180 (PSSPDHESPSQESPNAPTST) of human NLRP1/NALP1 was used as immunogen, GenBank no. NP_127497.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
• Control:

  NLRP1/NALP1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30930743).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for NLRP1/NALP1 Antibody

• CARD7
• CARD7SLEV1
• Caspase recruitment domain-containing protein 7
• CLR17.1
• Death effector filament-forming ced-4-like apoptosis protein
• DEFCAP
• DEFCAPVAMAS1
• DKFZp586O1822
• KIAA0926DEFCAP-L/S
• NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
• NACHT, leucine rich repeat and PYD containing 1
• NACHT, LRR and PYD containing protein 1
• NACHT, LRR and PYD domains-containing protein 1
• NACPP1044
• NALP1
• NALP1caspase recruitment domain protein 7
• NLR family, pyrin domain containing 1
• NLRP1
• Nucleotide-binding domain and caspase recruitment domain
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1
• PP1044
• SLEV1
• VAMAS1

Background

NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. This antibody recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at approx. 166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NLRP1/NALP1 Antibody (NB100-56148)(3)

Publications using NB100-56148

• Xu L, Peng F, Luo Q, Ding Y et Al. IRE1? silences dsRNA to prevent taxane-induced pyroptosis in triple-negative breast cancer Cell 2024-10-17 [PMID: 39419025]
• Ketelauri P, Scharov K, von Gall C et Al. Acute Circadian Disruption Due to Constant Light Promotes Caspase 1 Activation in the Mouse Hippocampus Cells 2023-07-12 [PMID: 37508501]
• Pronin A, Pham D, An W et al. Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury Front Mol Neurosci 2019-03-13 [PMID: 30930743] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: NLRP1
• Entrez:
  • 22861(Human)